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PURIFICATION
Do you know what the Isoelectric point and Mr of E.coli proteins is?
What are the best detergents for membrane protein solubilisation?
Do you know which Purification Strategy you should use?
What are the most common Affinity/Solubility Tags?
Nickel vs Cobalt. Which metal is best for IMAC?
Which His Tag Length, Position or Sequence to use?
Everything you need to know about Cleavage Enzymes
How do you stop your antibody from precipitating out of solution?
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