Picking the Right Expression System

HEK vs CHO for Protein Production

HEK CHO
293 cells are highly transfectable with most transfection reagents Suspension growth in serum-free media
Growth attached in basic media or suspension (requiring more expensive, protein-free media) 30-50% less protein than HEK
Protein Expression for structural studies eg X-ray crystallography Lower transfection efficiency
Choice for biopharmaceuticals = GMP validated

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