Superior Broth

Since the production of recombinant proteins can vary significantly depending on the type of medium employed, we recommend using the Media Optimization Kit™ to find out which medium is best for your protein.

Superior Broth™, Superior Prime Broth™, and new Superior Prime-olate™, are proprietary media formulations developed for improved expression of recombinant proteins in E. coli. The formulations are complex, supplying a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2 ± 0.2 to prevent acidification of the medium during the metabolism of the glucose.

Expression of recombinant proteins should be induced at cell densities twice that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth. Superior Broth™ shows the highest plasmid copy numbers among our family of media. This medium is an excellent choice for large-scale plasmid preparations. Biomass yields will depend on the process and the strain employed.

superior broth