SILAC (Stable isotope labelling with amino acids in cell culture) media is prepared specifically for labeling experiments involving the use of stable amino acid isotopes. Ideal for NMR (Nuclear Magnetic Resonance) or Mass Spec experiments for large scale proteomics.
We provide several of the most commonly used media without L-arginine, L-lysine, L-leucine and L-methionine, the four most commonly used heavy isotope-labelled amino acids. The SILAC medium is sterile filled in 500 ml bottles. Stock solutions of each of the four non-labelled amino acids are provided with each bottle of the deficient medium to permit reconstitution of the complete medium.
- Ready-to-use with L-Glutamine
- 7 Common Formulations
- Custom Formulations Available
A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)”,Shao-En Ong & Matthias Mann., Nature Protocols 1, – 2650 – 2660 (2007)