Super Cobalt NTA Affinity Resin was developed for the affinity purification of proteins carrying a polyhistidine tag. This affinity chromatography matrix consists of 7.5% cross-linked agarose.
The material is highly porous to allow for optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes under low pressure with flow rates of up to 6 mL/min (optimal 0.5–2 mL/min).
Our agarose is very homogeneous in size with a medium particle diameter of 40 µm, yielding a high degree of reproducibility between individual purification runs. If required, the cobalt ions can be removed from the agarose matrix using 5 wash steps with 100 mM EDTA, and the matrix can be recharged with a different metal ion.
Super Cobalt NTA Affinity Resin is delivered as a 50% (v/v) suspension. Therefore, 2 mL suspension will yield a 1 mL bed volume. The suspension contains 20% ethanol to prevent microbial growth.
- Specificity – His-Tag
- Matrix – 7.5% cross-linked Agarose
- Binding capacity – 30 mg/ml
- Bead size range – 32-60 µm
- Maximum pressure – 72 psi
- Stability/storage – pH 2-14