Turbo Prime Broth™ (Powder Media) is a proprietary media formulation used for the cultivation of recombinant strains of E.coli. The media improve the yield of recombinant proteins and typically produce cell yields 4 to 5 times higher than that of LB Broth in shake flask cultures depending on the strain. They have higher plasmid copy numbers than LB, which can enhance expression. The medium uses glycerol for a carbon source and has a rich nutrient base of amino acids, vitamins, inorganic minerals and trace minerals. The media are buffered at pH 7.2 +/- 0.2 with potassium phosphate to prevent a change in pH and provide a source of phosphate.