Superior Prime Broth™ (Powder) is a proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The formulation is complex, supplying a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2 ± 0.2 to prevent acidification of the medium during metabolism of the glucose. Expression of recombinant proteins should be induced at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth.