Sphingosine 1-Phosphate Receptor 3 (extracellular) Antibody (Serotonin Receptor 1A), WB/FC, 0.2ml

£0.00

SKU: AG1013-200 Category: Tag:

Description

Sphingosine 1-Phosphate Receptor 3 (extracellular) Antibody is a highly specific antibody directed against an extracellular epitope of mouse sphingosine 1-phosphate receptor 3. Anti-Sphingosine 1-Phosphate Receptor 3 (extracellular) antibody (#AG1013) can be used in western blot analysis and indirect flow cytometry applications. It has been designed to recognize S1PR3 from human, rat and mouse samples.

Application
WB, FC
Primary Accession
Q9Z0U9
Reactivity
Human, Mouse, Rat
Calculated MW
42270Da
Homology
Rat – identical; human – 9/12 amino acid residues identical.
Gene ID
13610
Other Names
S1PR3; Edg3; Lysophospholipid receptor 3
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse Sphingosine 1-phosphate receptor 3 (Accession Q9Z0U9). Extracellular, N-terminus.
Dilution
WB~~1:200
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.8 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

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