Description
Contents
Qty: 4 x 1.6 ml Protein A Midi spin columns
Max. sample volume per load: 20 ml, swing bucket rotor
Collection tube: 8 x 20 ml centrifuge tubes
Min. number of purifications: 20 purifications (5 uses per column)
Typical capacity/preparation: 20 mg human IgG
Vivaspin 20 ultrafiltration concentrators: 4
Buffers: 1 x Binding Buffer A (250 ml), 1 x Elution Buffer pH 5.5 B1 (125 ml), 1 x Elution Buffer pH 2.5 B2 (125 ml), 1 x Neutralization Buffer pH 9.0 C (30 ml)
Downloads
Proteus Protein A & G Brochure
Proteus Protein A Handbook
Proteus Protein A Midi Card
Dr Khalili –
I used this kit to purify Fabs from Fc fragment and other undigested IgG. I needed to have endotoxin free Fabs after digesting the full IgG which was not possible with other protein A kits. The Protein A Midi kit is very fast and efficient to obtain endotoxin free Fabs. I highly recommend this product.