Description
Product Name |
Optima PRO De-salting Kit (10 plates) |
Protein Application |
Removal of salts, low molecular weight impurities (e.g. imidazole) and buffer exchange |
DNA Application |
Removal of DNA primers and fragments (up to 20 bases), buffers, nucleotides labelled with biotin, isotopes and other markers |
Plate format |
96 well |
Mode of operation |
Centrifugation (bench-top or floor model) Positive pressure e.g. Tecan Resolvex A200 |
Total processing time |
Centrifugation ? 8mins Tecan ResolveX A200 ? 2mins |
Optimal Centrifugation Conditions |
900 x g |
Gel Matrix |
Zetadex G50 resin |
Gel bed volume |
800 _l |
Removal of Salt: (200 _l 1M NaCl) |
Up-to 99% |
For processing proteins* |
> 5 kDa |
Protein Recovery |
> 90% |
Maximum sample volume |
300 _l |
Minimum sample volume |
50 _l |
Plastic |
Polypropylene |
Storage buffer |
20 % Ethanol |
Working pH |
2 ? 13 |
Storage conditions |
4?C |
Expiry |
2 ? 3 years |
Catalogue Number |
PRO96 |
Pack sizes |
2, 10 and 50 de-salting plates |
* Smaller proteins may need to be centrifuged longer in order to elute
Kit Components:
10 x Optima PRO De-salting plate
10 x 0.35 ml Capillary (collection) plate
10 x 0.5 ml Waste plate
10 x Plate lid
Downloads
Optima PRO De-salting Plate Brochure
Optima PRO De-salting Plate Instructions for Use
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