Fastback Ni Advance Resin (25 ml)

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SKU: Fastback-Ni-Adv-25 Category: Tag:

Description

Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant. Nickel ions are carefully loaded onto an agarose matrix via chelating coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues. 

Benefits

– Ideal for secreted proteins and all intracellular protein expression

– Works in your protocol, regardless of sample origin whether eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)

– Great results faster with fewer steps than conventional workflow – no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column. 

– Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).

Downloads

Fastback Nickel Adv Resin Datasheet
Protein Ark HiFliQ Ni Adv Resin SDS

Specifications

Specificity Polyhistidine tag
Matrix 6% cross-linked agarose
Coupled ligand Chelating ligand
Binding capacity 80 mg/ml
Bead size 90 µm
Metal ion capacity (Cu, Ni) > 75 µmol/ml
Flow rate 0.25 – 2 ml/min, max 6.0 ml/min
Maximum pressure 45 psi
DTT stability (24 hours) 20 mM
EDTA stability (24 hours) 20 mM
Buffer compatibility Common aqueous buffers from pH 4-9
Shipping/delivery Ambient temperature
Storage

Equilibration buffer at 2-8ºC (short-term)

20% ethanol at 2-8ºC (long-term)

Order Information


Description Volume Product code
Fastback Ni Advance Resin (10 ml) 10 ml Fastback-Ni-Adv-10
Fastback Ni Advance Resin (25 ml) 25 ml Fastback-Ni-Adv-25
Fastback Ni Advance Resin (100 ml) 100 ml Fastback-Ni-Adv-100


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