Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant. Nickel ions are carefully loaded onto an agarose matrix via chelating coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues.
– Ideal for secreted proteins and all intracellular protein expression
– Works in your protocol, regardless of sample origin whether eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)
– Great results faster with fewer steps than conventional workflow – no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column.
– Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).
|Matrix||6% cross-linked agarose|
|Coupled ligand||Chelating ligand|
|Binding capacity||80 mg/ml|
|Bead size||90 µm|
|Metal ion capacity (Cu, Ni)||> 75 µmol/ml|
|Flow rate||0.25 – 2 ml/min, max 6.0 ml/min|
|Maximum pressure||45 psi|
|DTT stability (24 hours)||20 mM|
|EDTA stability (24 hours)||20 mM|
|Buffer compatibility||Common aqueous buffers from pH 4-9|
Equilibration buffer at 2-8ºC (short-term)
20% ethanol at 2-8ºC (long-term)
|Fastback Ni Advance Resin (10 ml)||10 ml||Fastback-Ni-Adv-10|
|Fastback Ni Advance Resin (25 ml)||25 ml||Fastback-Ni-Adv-25|
|Fastback Ni Advance Resin (100 ml)||100 ml||Fastback-Ni-Adv-100|