ACES™ Signal Sequence Kit


1 kit


ACES™ Signal Sequence Kit  provides a set of six expression vectors each with a different signal sequence for protein export to the periplasmic space of E. coli using either the SEC, SRP or TAT protein secretion pathway. Since the use of a post translational or co-translational export mechanism is protein-specific and cannot be known a priori, this kit allows for the selection of the translocation pathway best suited for a given recombinant protein. Expression is from the T5 promoter under the control of the lacoperator. Protein production is initiated by the addition of IPTG or lactose (only with Lac+ strains) to the culture medium.


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