A2B Adenosine Receptor (extracellular) Antibody (Adenosine A2B Receptor), WB/IHC/FC, 0.2ml

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SKU: AG1385-200 Category: Tag:

Description

The Anti-A2B-Adenosine Receptor (extracellular) antibody (#AG1385) can be used for western blot immunohistochemical applications. It has been designed to recognize A2B-Adenosine receptor from human, rat and mouse samples.

Application
WB, IHC, FC
Primary Accession
P29275
Reactivity
Human, Mouse, Rat
Calculated MW
36333 Da
Homology
Rat – 14/19 amino acid residues identical; mouse – 13/19 amino acid residues identical
Gene ID
136
Other Names
Adenosine A2B Receptor
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2B Adenosine Receptor with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). 2nd extracellular loop.
Dilution
WB~~1:200
IHC~~1:50
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.75 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

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