Description
The Anti-A2B-Adenosine Receptor (extracellular) antibody (#AG1385) can be used for western blot immunohistochemical applications. It has been designed to recognize A2B-Adenosine receptor from human, rat and mouse samples.
| Application WB, IHC, FC |
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| Primary Accession P29275 |
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| Reactivity Human, Mouse, Rat |
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| Calculated MW 36333 Da |
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| Homology Rat – 14/19 amino acid residues identical; mouse – 13/19 amino acid residues identical |
| Gene ID 136 |
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| Other Names Adenosine A2B Receptor |
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| Related products for control experiments Control peptide antigen (supplied with the antibody free of charge). |
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| Target/Specificity Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2B Adenosine Receptor with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). 2nd extracellular loop. |
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| Dilution WB~~1:200 IHC~~1:50 |
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| Peptide Confirmation Confirmed by amino acid analysis. |
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| Format Affinity purified antibody, lyophilized powder |
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| Reconstitution 50 ?l or 0.2 ml deionized water, depending on the sample size. |
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| Antibody Concentration After Reconstitution 0.75 mg/ml. |
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| Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
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| Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
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| Storage After Reconstitution The reconstituted solution can be stored at 4?C for up to 2 weeks. For longer periods, small aliquots should be stored at -20?C or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 _ g 5 min). |
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| Control Antigen Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
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| Control Antigen Reconstitution 100 ?l water. |
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| Control Antigen Storage After Reconstitution -20?C. |
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| Preadsorption Control 1 ?g peptide per 1 ?g antibody. |
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