Description
Anti-A2A Adenosine Receptor antibody (#AG1386) can be used in western blot and immunohistochemical applications, and has been designed to recognize A2AAR from human, rat, and mouse samples.
| Application WB, IHC |
|
| Primary Accession P60613 |
|
| Reactivity Human, Mouse, Rat |
|
| Calculated MW 44971 Da |
|
| Homology Rat, human and canis identical |
| Gene ID 11540 |
|
| Other Names Adenosine A2A Receptor, ADORA2a |
|
| Related products for control experiments Control peptide antigen (supplied with the antibody free of charge). |
|
| Target/Specificity Peptide (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse A2A Adenosine receptor (Accession Q60613). 3rd intracellular loop. |
|
| Dilution WB~~1:200 |
|
| Peptide Confirmation Confirmed by amino acid analysis. |
|
| Format Affinity purified antibody, lyophilized powder |
|
| Reconstitution 50 ?l or 0.2 ml deionized water, depending on the sample size. |
|
| Antibody Concentration After Reconstitution 0.75 mg/ml. |
|
| Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
|
| Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
|
| Storage After Reconstitution The reconstituted solution can be stored at 4?C for up to 2 weeks. For longer periods, small aliquots should be stored at -20?C or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 _ g 5 min). |
|
| Control Antigen Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
|
| Control Antigen Reconstitution 100 ?l water. |
|
| Control Antigen Storage After Reconstitution -20?C. |
|
| Preadsorption Control 1 ?g peptide per 1 ?g antibody. |
Reviews
There are no reviews yet.