A2A Adenosine Receptor Antibody (Adenosine A2A Receptor/ADORA2a), WB/IHC, 0.2ml

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SKU: AG1386-200 Category: Tag:

Description

 

Anti-A2A Adenosine Receptor antibody (#AG1386) can be used in western blot and immunohistochemical applications, and has been designed to recognize A2AAR from human, rat, and mouse samples.

Application
WB, IHC
Primary Accession
P60613
Reactivity
Human, Mouse, Rat
Calculated MW
44971 Da
Homology 
Rat, human and canis identical
Gene ID
11540
Other Names
Adenosine A2A Receptor, ADORA2a
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide (C)RQLKQMESQPLPGER,  corresponding to amino acid residues 201-215 of  mouse A2A Adenosine receptor (Accession Q60613). 3rd intracellular loop.
Dilution
WB~~1:200
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.75 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

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