5-Hydroxytryptamine Receptor 3B (extracellular) Antibody (Serotonin receptor 3B), WB, 0.2ml

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SKU: AG1008-200 Category: Tag:

Description

5-Hydroxytryptamine Receptor 3B (5-HT3B) belongs to the super-family of ligand-gated ion channels. Serotonin receptors, other than 5-HT3 subtype belong to the super-family of G-protein coupled receptors.
 

Application
WB
Primary Accession
Q9JJ16
Reactivity
Mouse, Rat
Calculated MW
50336 Da
Homology
Mouse – identical. Human- not recommended for use with human samples.
Gene ID
58963
Other Names
Serotonin receptor 3B; 5-HT3B
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine Receptor 3B (Accession Q9JJ16). Extracellular, N-terminus.
Dilution
WB~~1:200
Peptide Confirmation
Confirmed by amino acid analysis and mass-spectrography.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 ml or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.95 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

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