5-Hydroxytryptamine Receptor 3A Antibody (Serotonin receptor 3A), WB, 0.2ml

£0.00

SKU: AG1009-200 Category: Tag:

Description

5-HT3 receptors have become important targets for which to develop treatments regarding irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder.

Application
WB
Primary Accession
P35563
Reactivity
Mouse, Rat
Calculated MW
55428 Da
Homology
Mouse – identical; human – 13/14 amino acid residues identical.
Gene ID
79246
Other Names
5-HT3A, Serotonin receptor 3A
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine Receptor 3A (Accession P35563). 2nd intracellular loop.
Dilution
WB~~1:200
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.8 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Menu