Description
5-HT3 receptors have become important targets for which to develop treatments regarding irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder.
| Application WB |
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| Primary Accession P35563 |
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| Reactivity Mouse, Rat |
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| Calculated MW 55428 Da |
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| Homology Mouse – identical; human – 13/14 amino acid residues identical. |
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| Gene ID 79246 |
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| Other Names 5-HT3A, Serotonin receptor 3A |
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| Related products for control experiments Control peptide antigen (supplied with the antibody free of charge). |
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| Target/Specificity Peptide (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine Receptor 3A (Accession P35563). 2nd intracellular loop. |
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| Dilution WB~~1:200 |
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| Peptide Confirmation Confirmed by amino acid analysis. |
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| Format Affinity purified antibody, lyophilized powder |
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| Reconstitution 50 ?l or 0.2 ml deionized water, depending on the sample size. |
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| Antibody Concentration After Reconstitution 0.8 mg/ml. |
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| Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
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| Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
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| Storage After Reconstitution The reconstituted solution can be stored at 4?C for up to 2 weeks. For longer periods, small aliquots should be stored at -20?C or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 _ g 5 min). |
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| Control Antigen Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20?C. |
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| Control Antigen Reconstitution 100 ?l water. |
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| Control Antigen Storage After Reconstitution -20?C. |
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| Preadsorption Control 1 ?g peptide per 1 ?g antibody. |
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