5-Hydroxytryptamine Receptor 1B (extracellular) Antibody (Serotonin receptor 1B), WB, 0.2ml

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SKU: AG1011-200 Category: Tag:

Description

5-Hydroxytryptamine Receptor 1B (extracellular) Antibody  is a highly specific Antibody directed against an extracellular epitope of human  5-Hydroxytryptamine Receptor 1B (5-HT1B). It can be used for Western blot analysis and has been  designed to recognize 5-Hydroxytryptamine Receptor 1B from human, rat and mouse samples.

Application
WB
Primary Accession
P28222
Reactivity
Rat
Calculated MW
43568 Da
Homology
Rat – 15/17 amino acid residues identical; mouse – 14/17 amino acid residues identical.
Gene ID
3351
Other Names
5-HT1B, Serotonin receptor 1B
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine Receptor 1B (Accession P28222). Extracellular, N-terminus.
Dilution
WB~~1:400
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.8 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

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