| Checklist to Improve pre-cast of protein gels | Check |
| Tailor your SDS-gel to your sample | ✓ |
| Use a compatible Running Buffer for your gel type. Every pre-cast protein gel is optimized for a specific running buffer | ✓ |
| Use a compatible sample loading buffer | ✓ |
| Prepare the sample correctly; ↑ Heating Time = ↓ Resolution; Control= 3 minutes at 100°C | ✓ |
| For membrane proteins, avoid boiling the samples; remember that membrane proteins can irreversibly aggregate in SDS at temperatures >50°C | ✓ |