Since the production of recombinant proteins can vary significantly depending on the type of medium employed, we recommend using the Media Optimization Kit™ to find out which medium is best for your protein.
Power Broth™, Power Prime Broth™, and Power Prime-olate™ are proprietary media formulations developed for improved expression of recombinant proteins in E. coli. These media are composed of a complex, rich mixture of amino acids, vitamins, and a carbon source at higher levels than those of Terrific Broth. The media are buffered at pH 6.8 ± 0.2. Power Prime™ and Power Prime-olate™ are APF Certified™.
For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Several reports suggest that Power Broth™ and Power Prime Broth™ can increase the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is suitable as the base for fermentation cultures. Biomass yields will depend on the process and the strain employed.
