Which His Tag Length, Position or Sequence to use?
Length and position of HisTags can affect the following:
| Expression Yield |
| Efficiency of translation. Triplet codons located after ATG can inhibit translation. Use highest expressing AAA (Lysine) at position 2 |
| Binding to IMAC resin |
| Results (activity, crystallization, NMR …etc). Remove the tag! |
| Oligomerization state |
E.coli water channel AqpZ expression and purification using different His-Tags (Co = cobalt-IMAC, & subsequent Q2= ion-exchange)
