Which His Tag Length, Position or Sequence to use?
Length and position of HisTags can affect the following:
Expression Yield |
Efficiency of translation. Triplet codons located after ATG can inhibit translation. Use highest expressing AAA (Lysine) at position 2 |
Binding to IMAC resin |
Results (activity, crystallization, NMR …etc). Remove the tag! |
Oligomerization state |