Checklist to Improve pre-cast of protein gels Check Tailor your SDS-gel to your sample ✓ Use a compatible Running Buffer for your gel type. Every pre-cast protein gel is optimized… read more →
Rat and some mouse IgGs can be prone to precipitation. Neutralize half of the eluate in neutralisation buffer pH 9.0. Dialyze the other half twice in 100 x volume of PBS overnight.