How to Improve Pre-Cast Protein Gels?
|Checklist to Improve pre-cast of protein gels||Check|
|Tailor your SDS-gel to your sample||✓|
|Use a compatible Running Buffer for your gel type. Every pre-cast protein gel is optimized for a specific running buffer||✓|
|Use a compatible sample loading buffer||✓|
|Prepare the sample correctly; ↑ Heating Time = ↓ Resolution; Control= 3 minutes at 100°C||✓|
|For membrane proteins, avoid boiling the samples; remember that membrane proteins can irreversibly aggregate in SDS at temperatures >50°C||✓|