How to Improve Pre-Cast Protein Gels?

Checklist to Improve pre-cast of protein gels Check
Tailor your SDS-gel to your sample  
Use a compatible Running Buffer for your gel type. Every pre-cast protein gel is optimized for a specific running buffer  ✓
Use a compatible sample loading buffer  ✓
Prepare the sample correctly; ↑ Heating Time = ↓ Resolution; Control= 3 minutes at 100°C  ✓
For membrane proteins, avoid boiling the samples; remember that membrane proteins can irreversibly aggregate in SDS at temperatures >50°C  ✓