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400 different detergent (non-ionic, ionic, Zwitterionic)
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Critical micelle concentration (CMC) concentration (0.3-10x CMC typically used)
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Ionic strength of the solution (100-200 mM NaCl typically used)
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Purity (α-isomer content)
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Micelle size and shape
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Compatibility with purification techniques
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Removal techniques (dialysis, ion-exchange, size-exclusion chromatography)
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Mixed detergents eg Cholesterol & CHS / exchanging detergents
Properties of Non-Ionic Detergents
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60% of structures are solved with non-ionic detergents: DDM, DM, NG, OG, LDAO
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DDM remains the defacto detergent of choice for many membrane protein extractions.
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