How to make your Bacterial Expression More Soluble?

Cell line optimization to increase soluble expression
Cell Line Details
BL21 (DE3) Standard IPTG expression
Cell Line Details
Turner™ (DE3) Lac permease (LacY) deletion: IPTG concentration dependent expression
Lemo21 (DE3) L-rhamnose induces expression of lysozyme (lysY) which inhibits T7 RNA polymerase
Cell Line Details
BL21-CodonPlus(DE3)-RIL Contains tRNAs for codons AGA, AGG, the isoleucine codon AUA, and the leucine codon CUA
Rosetta™ (DE3) Contains tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons
Cell Line Details
Origami™ (DE3) Mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor)
Cell Line Details
Rosetta-gami™ Combined Rosetta™ & Origami™
Cell Line Details
ArcticExpress™ Low growth temperatures & expresses chaperonins Cpn60 and Cpn10 from O. Antarctica
BL21 (DE3) Co-expression with molecular chaperones (groES-groEL or dnaK-dnaJ-grpE-groES-groEL)
Cell Line Details
C41 (DE3) Contains mutations to prevents cell death from toxic proteins
C43 (DE3) C41 (DE3) with F-ATPase subunit gene
Cell Line Details
ClearColi™ Mutations produce a substitute non-toxic molecule on the outer membrane. Other mutations prevent Lipopolysaccharide (LPS) production.