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How do you stop your antibody from precipitating out of solution?
How do you stop your antibody from precipitating out of solution?
18/06/2017
Protein Ark
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Purification
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Tips
Rat and some mouse IgGs can be prone to precipitation. Neutralize half of the eluate in neutralisation buffer pH 9.0. Dialyze the other half twice in 100 x volume of PBS overnight.
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The latest Lab tips from Mike and Alex. Feel free to send your own lab tips and tricks to info@proteinark.com and we will publish them on our website!
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