The Optima PRO De-salting Plate is designed for small-scale de-salting, buffer exchange and removal of low molecular weight materials (e.g. imidazole) from larger biomolecules, or macromolecules such as proteins. Optima PRO de-salting plates are gel filtration plates consisting of 800 µl bed volume in a standardized array for processing Protein samples >5kDa. Optima PRO de-salting plate can be used for the removal of DNA primers and fragments (up to 20 bases), nucleotides labelled with biotin, isotopes, and other assorted markers.
The Optima PRO De-salting Plate provides convenient small-scale preparation of protein samples post affinity purification, or prior to analysis techniques such as gel electrophoresis, liquid chromatography and LC-MS. Designed for imidazole removal, desalting and buffer exchange.
| Product Name | Optima PRO De-salting Kit |
| Protein Application | Removal of salts, low molecular weight impurities (e.g: imidazole) and buffer exchange |
| DNA Application | Removal of DNA primers and fragments (up to 20 bases), buffers, nucleotides labelled with biotin, isotopes and other markers |
| Plate Format | 96 well |
| Mode of Operation | Centrifugation (bench-top or floor model) Positive Pressure (e.g: Tecan Resolvex A200) |
| Total Processing Time | Centrifugation – 8mins Tecan ResolveX A200 – 2mins |
| Optimal Centrifugation Conditions | 900 x g |
| Gel Matrix | Zetadex G50 resin |
| Gel Bed Volume | 800 µl |
| Removal of Salt: (200 μl 1M NaCl) | Up to 99% |
| For Processing Proteins* | > 5 kDa |
| Protein Recovery | > 90% |
| Maximum Sample Volume | 300 µl |
| Minimum Sample Volume | 50 µl |
| Plastic | Polypropylene |
| Storage Buffer | 20 % Ethanol |
| Working pH | 2-13 |
| Storage Conditions | 4°C |
| Expiry | 2-3 years |
| Pack Sizes | 2, 10 and 50 de-salting plates |
*Smaller proteins may need to be centrifuged longer in order to elute.
