The Optima PRO De-salting Plate is designed for small-scale de-salting, buffer exchange and removal of low molecular weight materials (e.g. imidazole) from larger biomolecules, or macromolecules such as proteins. Optima PRO de-salting plates are gel filtration plates consisting of 800 µl bed volume in a standardized array for processing Protein samples >5kDa. Optima PRO de-salting plate can be used for the removal of DNA primers and fragments (up to 20 bases), nucleotides labelled with biotin, isotopes, and other assorted markers.
The Optima PRO De-salting Plate provides convenient small-scale preparation of protein samples post affinity purification, or prior to analysis techniques such as gel electrophoresis, liquid chromatography and LC-MS. Designed for imidazole removal, desalting and buffer exchange.
|Product Name||Optima PRO De-salting Kit|
|Protein Application||Removal of salts, low molecular weight impurities (e.g: imidazole) and buffer exchange|
|DNA Application||Removal of DNA primers and fragments (up to 20 bases), buffers, nucleotides labelled with biotin, isotopes and other markers|
|Plate Format||96 well|
|Mode of Operation||Centrifugation (bench-top or floor model)
Positive Pressure (e.g: Tecan Resolvex A200)
|Total Processing Time||Centrifugation – 8mins
Tecan ResolveX A200 – 2mins
|Optimal Centrifugation Conditions||900 x g|
|Gel Matrix||Zetadex G50 resin|
|Gel Bed Volume||800 µl|
|Removal of Salt: (200 μl 1M NaCl)||Up to 99%|
|For Processing Proteins*||> 5 kDa|
|Protein Recovery||> 90%|
|Maximum Sample Volume||300 µl|
|Minimum Sample Volume||50 µl|
|Storage Buffer||20 % Ethanol|
|Pack Sizes||2, 10 and 50 de-salting plates|
*Smaller proteins may need to be centrifuged longer in order to elute.