Product Code: AG1010-200
Availability: In Stock

Qty

Icon Ask about this product

5-HT3 receptors have become important targets for which to develop treatments regarding irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder.

Application
WB
Primary Accession
P28565
Reactivity
Human, Rat
Calculated MW
41540 Da
Homology
Mouse - identical; human - 13/14 amino acid residues identical.
Gene ID
25323
Other Names
5-HT1D; Serotonin receptor 1D
Related products for control experiments
Control peptide antigen (supplied with the antibody free of charge).
Target/Specificity
Peptide (C)EASNRSLNATGAWD, corresponding to amino acid residues  14-27 of rat 5-hydroxytryptamine receptor 1D  (Accession P28565. Extracellular, N-terminus.
Dilution
WB~~1:200
Peptide Confirmation
Confirmed by amino acid analysis.
Format
Affinity purified antibody, lyophilized powder
Reconstitution
50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution
0.8 mg/ml.
Buffer After Reconstitution
Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After Reconstitution
The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before Reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution
100 µl water.
Control Antigen Storage After Reconstitution
-20ºC.
Preadsorption Control
1 µg peptide per 1 µg antibody.

 

Write a review

Note: HTML is not translated!