Optima PRO De-salting Plate

The Optima PRO de-salting plate is designed for small-scale de-salting, buffer exchange and removal of low molecular weight materials (e.g. imidazole) from larger biomolecules, or macromolecules such as proteins. Optima PRO de-salting plates are gel filtration plates consisting of 800 μL bed volume in a standardized array for processing Protein samples >5kDa. Optima PRO de-salting plate can be used for the removal of DNA primers and fragments (up to 20 bases), nucleotides labelled with biotin, isotopes, and other assorted markers. 


The Optima PRO de-salting plate provides convenient small-scale preparation of protein samples post affinity purification, or prior to analysis techniques such as gel electrophoresis, liquid chromatography and LC-MS. Designed for imidazole removal, desalting and buffer exchange.



Product Name

Optima PRO De-salting Kit

Protein Application

Removal of salts, low molecular weight impurities (e.g. imidazole) and buffer exchange

DNA Application

Removal of DNA primers and fragments (up to 20 bases), buffers, nucleotides labelled with biotin, isotopes and other markers

Plate format

96 well

Mode of operation

Centrifugation (bench-top or floor model)

Positive pressure e.g. Tecan Resolvex A200

Total processing time

Centrifugation – 8mins

Tecan ResolveX A200 – 2mins

Optimal Centrifugation Conditions

900 x g

Gel Matrix

Zetadex G50 resin

Gel bed volume

800 μl

Removal of Salt: (200 μl 1M NaCl)

Up-to 99% 

For processing proteins*

> 5 kDa

Protein Recovery

> 90%

Maximum sample volume

300 μl

Minimum sample volume

50 μl

Plastic

Polypropylene

Storage buffer

20 % Ethanol

Working pH

2 – 13  

Storage conditions

4°C

Expiry

2 – 3 years 

Catalogue Number

PRO96

Pack sizes

2, 10 and 50 de-salting plates

* Smaller proteins may need to be centrifuged longer in order to elute


Downloads

Optima PRO De-salting Plate Brochure
Optima PRO De-salting Plate Instructions for Use



Optima PRO De-salting kit (10 plates)
Product NameOptima PRO De-salting Kit (10 plates)Protein ApplicationRemoval of salts, low molecular ..
Optima PRO De-salting kit (2 plates)
Product NameOptima PRO De-salting Kit (2 plates)Protein ApplicationRemoval of salts, low molecular w..
Optima PRO De-salting Plates (10 plates only)
Product NameOptima PRO De-salting Plates (10 plates only)Protein ApplicationRemoval of salts, low mo..
Optima PRO De-salting Plates (50 plates)
Product NameOptima PRO De-salting Plates (50 plates)Protein ApplicationRemoval of salts, low molecul..
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