Fastback Ni Advance Resin

Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant. Nickel ions are carefully loaded onto an agarose matrix via chelating coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues. 

Benefits

- Ideal for secreted proteins and all intracellular protein expression

- Works in your protocol, regardless of sample origin whether eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)

- Great results faster with fewer steps than conventional workflow - no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column. 

- Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).


Specifications

SpecificityPolyhistidine tag
Matrix6% cross-linked agarose
Coupled ligandChelating ligand
Binding capacity80 mg/ml
Bead size90 µm
Metal ion capacity (Cu, Ni)> 75 µmol/ml
Flow rate0.25 - 2 ml/min, max 6.0 ml/min
Maximum pressure45 psi
DTT stability (24 hours)20 mM
EDTA stability (24 hours)20 mM
Buffer compatibilityCommon aqueous buffers from pH 4-9
Shipping/deliveryAmbient temperature
Storage

Equilibration buffer at 2-8ºC (short-term)

20% ethanol at 2-8ºC (long-term)

Order Information


DescriptionVolumeProduct code
Fastback Ni Advance Resin (10 ml)10 mlFastback-Ni-Adv-10
Fastback Ni Advance Resin (25 ml)
25 mlFastback-Ni-Adv-25
Fastback Ni Advance Resin (100 ml)
100 mlFastback-Ni-Adv-100

Downloads

Fastback Nickel Adv Resin Datasheet
Protein Ark HiFliQ Ni Adv Resin SDS
Fastback Ni Advance Resin (10 ml)
Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture s..
Fastback Ni Advance Resin (25 ml)
Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture s..
Fastback Ni Advance Resin (100 ml)
Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture s..
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