Immobilized Metal Affinity Chromatography

Metal Ion | Nickel or Cobalt |
Ligand | IDA (iminodiacetic acid) and NTA (nitrilotriacetic acid) |
Capacity | Ni-NTA > Co-NTA > Ni-IDA |
Affinity | Ni2+ > Co2+ |
Specificity | Co2+ > Ni2+ |
Imidazole Elution | 250 mM (Ni) > 150 mM (Co) |
EDTA/DTT Resistance | NTA > IDA |
Re-chargeable | NTA > IDA |