Irreversible Thiol-coupling SepFast can be used for the specific immobilization of thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or equipment is required.
Protein Ark’s Fastback Irreversible Thiol-coupling High Flow media forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm that facilitates much improved ligand accessibility. This pre-activated agarose based resin can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.