Epoxy-activated agarose beads can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. They can also be used for the coupling of primary amine-containing, or thiol-containing molecules.
Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable.
Protein Ark’s Fastback Epoxy activated High Flow Resin is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.