Carboxyl-activated agarose beads can be used for the immobilization of amine containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well- documented technique.
Protein Ark’s Fastback Carboxyl-activated 4 High Flow (4HF) Resin forms chemically stable amide linkages with ligands containing primary amine groups. It contains a 9 atom spacer. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large-scale purification applications.