Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique.
Protein Ark’s Fastback Amine-activated High Flow Resin forms a chemically stable amide linkage with ligands containing carboxyl groups. It contains a 9 atom spacer. The pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large-scale purification applications.