The revolutionary Proteus spin columns are convenient and easy to use to purify your His-tagged fusion proteins. Unlike any other centrifugal column, Proteus contains a unique flow regulator, which is integrated in the resin. Flow rate is inextricably linked to the binding capacity of an affinity column. The flow regulator ensures that the column exerts a back pressure, slowing down the flow of the sample through the column. In this way, you achieve the yields and purities of a gravity column with the speed of a spin column.