Cell line optimization to increase soluble expression
| Cell Line | Details |
| BL21 (DE3) | Standard IPTG expression |
| Cell Line | Details |
| Turner™ (DE3) | Lac permease (LacY) deletion: IPTG concentration dependent expression |
| Lemo21 (DE3) | L-rhamnose induces expression of lysozyme (lysY) which inhibits T7 RNA polymerase |
| Cell Line | Details |
| BL21-CodonPlus(DE3)-RIL | Contains tRNAs for codons AGA, AGG, the isoleucine codon AUA, and the leucine codon CUA |
| Rosetta™ (DE3) | Contains tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons |
| Cell Line | Details |
| Origami™ (DE3) | Mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) |
| Cell Line | Details |
| Rosetta-gami™ | Combined Rosetta™ & Origami™ |
| Cell Line | Details |
| ArcticExpress™ | Low growth temperatures & expresses chaperonins Cpn60 and Cpn10 from O. Antarctica |
| BL21 (DE3) | Co-expression with molecular chaperones (groES-groEL or dnaK-dnaJ-grpE-groES-groEL) |
| Cell Line | Details |
| C41 (DE3) | Contains mutations to prevents cell death from toxic proteins |
| C43 (DE3) | C41 (DE3) with F-ATPase subunit gene |
| Cell Line | Details |
| ClearColi™ | Mutations produce a substitute non-toxic molecule on the outer membrane. Other mutations prevent Lipopolysaccharide (LPS) production. |