ACES™ (AthenaES™ Complete Expression System): Signal Sequence Kit The ACES™ (AthenaES™ Complete Expression System) Signal Sequence Kit provides a set of six expression vectors each with a different signal sequence for protein export to the periplasmic space of E. coli using either the SEC, SRP or TAT protein secretion pathway. Since the use of a post translational or co-translational export mechanism is protein-specific and cannot be known a priori, this kit allows for the selection of the translocation pathway best suited for a given recombinant protein. Expression is from the T5 promoter under the control of the lacoperator. Protein production is initiated by the addition of IPTG or lactose (only with Lac+ strains) to the culture medium.
ACES™ (AthenaES™ Complete Expression System): YebF Protein Export Kit The ACES™ (AthenaES™ Complete Expression System) YebF Protein Export Kit makes use of the YebF protein to transport other proteins through the inner and outer membranes of E. colicells. This permits the secretion of target proteins into the culture medium, simplifying downstream purification as well as allowing for the expression of toxic proteins and proteins that do not fold correctly in the E. colicytoplasm.